Title
The regulation of RhoA at focal adhesions by StarD13 is important for astrocytoma cell motility
Publication Date
2014
Journal Title
Exp Cell Res
Abstract
Malignant astrocytomas are highly invasive into adjacent and distant regions of the normal brain. Rho GTPases are small monomeric G proteins that play important roles in cytoskeleton rearrangement, cell motility and tumor invasion. In the present study, we show that the knock down of StarD13, a GTPase activating protein (GAP) for RhoA and Cdc42, inhibits astrocytoma cell migration through modulating focal adhesion dynamics and cell adhesion. This effect is mediated by the resulting constitutive activation of RhoA and the subsequent indirect inhibition of Rac. Using Total Internal Reflection Fluorescence (TIRF)-based Forster Resonance Energy Transfer (FRET), we show that RhoA activity localizes with focal adhesions at the basal surface of astrocytoma cells. Moreover, the knock down of StarD13 inhibits the cycling of RhoA activation at the rear edge of cells, which makes them defective in retracting their tail. This study highlights the importance of the regulation of RhoA activity in focal adhesions of astrocytoma cells and establishes StarD13 as a GAP playing a major role in this process. (C) 2013 Elsevier Inc All rights reserved.
Volume Number
321
Issue Number
2
Pages
109-122
Document Type
Article
EPub Date
2013/12/18
Status
Northwell Researcher
Facility
Northwell Health
Primary Department
Molecular Medicine
Additional Departments
Neurosurgery
PMID
DOI
10.1016/j.yexcr.2013.11.023