Title

Analysis of the released nuclear cytokine HMGB1 in human serum

Publication Date

2014

Journal Title

Methods Mol Biol

Abstract

A ubiquitous nuclear protein, the high-mobility group box 1 (HMGB1), is secreted by activated macrophages/monocytes and leaked passively from injured cells. HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Here, we describe a semiquantitative immuno-blotting method to measure the released HMGB1 in human serum, in comparison with a commercially available HMGB1 ELISA technique.

Volume Number

1172

Pages

13-25

Document Type

Article

EPub Date

2014/06/09

Status

Faculty

Facility

School of Medicine

Primary Department

Emergency Medicine

PMID

24908291

DOI

10.1007/978-1-4939-0928-5_2