Publication Date
2014
Journal Title
Lupus Sci Med
Abstract
OBJECTIVE: To compare the performance characteristics of cell-bound complement (C4d) activation products (CBCAPS) on erythrocyte (EC4d) and B cells (BC4d) with antibodies to double-stranded DNA (anti-dsDNA) and complement C3 and C4 in systemic lupus erythematosus (SLE). METHODS: The study enrolled 794 subjects consisting of 304 SLE and a control group consisting of 285 patients with other rheumatic diseases and 205 normal individuals. Anti-dsDNA and other autoantibodies were measured using solid-phase immunoassays while EC4d and BC4d were determined using flow cytometry. Complement proteins were determined using immunoturbidimetry. Disease activity in SLE was determined using a non-serological Systemic Lupus Erythematosus Disease Activity Index SELENA Modification. A two-tiered methodology combining CBCAPS with autoantibodies to cellular and citrullinated antigens was also developed. Statistical analyses used area under receiver operating characteristic curves and calculations of area under the curve (AUC), sensitivity and specificity. RESULTS: AUC for EC4d (0.82+/-0.02) and BC4d (0.84+/-0.02) was higher than those yielded by C3 (0.73+/-0.02) and C4 (0.72+/-0.02) (p
Volume Number
1
Issue Number
1
Pages
e000056
Document Type
Article
EPub Date
2014/11/15
Status
Faculty
Facility
School of Medicine
Primary Department
Rheumatology
PMID
DOI
10.1136/lupus-2014-000056