Title
Analysis of circulating HMGB1 in human serum
Publication Date
2020
Journal Title
Methods Mol Biol
Abstract
© Springer Science+Business Media, LLC, part of Springer Nature 2020. As a ubiquitous nuclear protein, high-mobility group box 1 (HMGB1) is constitutively expressed and can be actively secreted by macrophages/monocytes, as well as passively released from damaged cells following pathological injuries. Studies indicate that HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Although intracellular HMGB1 functions as a regulator of tumorigenesis, epigenetic anticancer agents or therapeutic γ-ray irradiation could also cause active secretion or passive release of HMGB1, enabling serum HMGB1 to serve as a biomarker for the diagnosis and therapy of various cancers. Here we describe a semiquantitative immune blotting method to measure HMGB1 in human serum, in comparison with a commercially available HMGB1 enzyme-linked immunosorbent assay (ELISA) technique.
Volume Number
2108
Pages
15 - 28
Document Type
Article
Status
Faculty; Northwell Researcher
Facility
School of Medicine; Northwell Health
Primary Department
Emergency Medicine
PMID
DOI
10.1007/978-1-0716-0247-8_2