Analysis of circulating HMGB1 in human serum

Publication Date

2020

Journal Title

Methods Mol Biol

Abstract

© Springer Science+Business Media, LLC, part of Springer Nature 2020. As a ubiquitous nuclear protein, high-mobility group box 1 (HMGB1) is constitutively expressed and can be actively secreted by macrophages/monocytes, as well as passively released from damaged cells following pathological injuries. Studies indicate that HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Although intracellular HMGB1 functions as a regulator of tumorigenesis, epigenetic anticancer agents or therapeutic γ-ray irradiation could also cause active secretion or passive release of HMGB1, enabling serum HMGB1 to serve as a biomarker for the diagnosis and therapy of various cancers. Here we describe a semiquantitative immune blotting method to measure HMGB1 in human serum, in comparison with a commercially available HMGB1 enzyme-linked immunosorbent assay (ELISA) technique.

Volume Number

2108

Pages

15 - 28

Document Type

Article

Status

Faculty, Northwell Researcher

Facility

School of Medicine; Northwell Health

Primary Department

Emergency Medicine

PMID

31939167

DOI

10.1007/978-1-0716-0247-8_2

For the public and Northwell Health campuses

Share

COinS