Publication Date
2020
Journal Title
J Lipid Res
Abstract
Phospholipids, including ether phospholipids, are composed of numerous isomeric and isobaric species that have the same backbone and acyl chains. This structural resemblance results in similar fragmentation patterns by collision-induced dissociation of phospholipids regardless of class, yielding complicated tandem mass spectrometry (MS/MS) spectra when isobaric species are analyzed together. Furthermore, the presence of isobaric species can lead to misassignment of species when made solely based on their molecular weights. In this study, we used normal-phase HPLC for ESI-MS/MS analysis of phospholipids from bovine heart mitochondria. Class separation by HPLC eliminates chances for misidentification of isobaric species from different classes of phospholipids. Chromatography yields simple MS/MS spectra without interference from isobaric species, allowing clear identification of peaks corresponding to fragmented ions containing monoacylglycerol backbone derived from losing one acyl chain. Using these fragmented ions, we characterized individual and isomeric species in each class of mitochondrial phospholipids, including unusual species such as phosphatidylserine containing an ether linkage and species containing odd-numbered acyl chains in cardiolipin, phosphatidylserine, phosphatidylinositol, and phosphatidylglycerol. We also characterized monolysocardiolipin and dilysocardiolipin, the least abundant, but nevertheless important mitochondrial phospholipids. The results clearly show the power of HPLC-MS/MS for identification and characterization of phospholipids including minor species.
Volume Number
61
Issue Number
12
Pages
1707-1719
Document Type
Article
Status
Faculty
Facility
School of Medicine
Primary Department
Molecular Medicine
PMID
DOI
10.1194/jlr.ra120001044