Title
Analysis of the released nuclear cytokine HMGB1 in human serum
Publication Date
2014
Journal Title
Methods Mol Biol
Abstract
A ubiquitous nuclear protein, the high-mobility group box 1 (HMGB1), is secreted by activated macrophages/monocytes and leaked passively from injured cells. HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Here, we describe a semiquantitative immuno-blotting method to measure the released HMGB1 in human serum, in comparison with a commercially available HMGB1 ELISA technique.
Volume Number
1172
Pages
13-25
Document Type
Article
EPub Date
2014/06/09
Status
Faculty
Facility
School of Medicine
Primary Department
Emergency Medicine
PMID
DOI
10.1007/978-1-4939-0928-5_2